miR-335-5p suppresses gastric cancer progression by targeting MAPK10

Abstract Background Recent studies have established the roles of microRNAs (miRNAs) in cancer progression. The aberrant expression miR-335-5p has been reported many cancers, including gastric (GC). In this study, precise GC as well molecular mechanisms underlying its effects, role target MAPK10, were evaluated. Methods Quantitative real-time PCR was used to evaluate levels cell lines and tissues. MTT colony formation assays detect proliferation, Transwell wound-healing invasion migration cells. correlation between cycle-related gene mitogen-activated protein kinase 10 (MAPK10) analyzed. addition, candidate evaluated by a luciferase reporter assay, qRT-PCR, western blotting. Results downregulated tissues lines. Furthermore, inhibited proliferation cells induced apoptosis. Additionally, arrested cycle at G1/S phase vitro. Levels MAPK10 correlated, directly targeted miR-335-5p. Conclusions These data suggest that is tumor suppressor acts via inhibit